Lebitso la sehlahisoa:β-NADPH
Lebitso le leng:β-NADPH|beta-Nicotinamide adenine dinucleotide 2'-phosphate fokotswa tetrasodium letsoai hydrate
Synonym: beta-NADPH; 2'-NADPH hydrate; Coenzyme II e fokolitse letsoai la tetrasodium; Dihydronicotinamide adenine dinucleotide phosphate tetrasodium letsoai; NADPH Na4; TPNH2 Na4; Triphosphopyridine nucleotide e fokolitse letsoai la tetrasodium
Nomoro ea CAS: 2646-71-1
Nomoro ea EINECS:220-163-3
Bohloeki:≥98%
Mocheso oa polokelo: -20°C
Ponahalo: Phofo e tšoeu ho isa ho e mosehla
Tlhaloso ea Sehlahisoa: β-NADPH (β-Nicotinamide Adenine Dinucleotide Phosphate, Sebopeho se Fokotseng sa Tetrasodium Letsoai)
Nomoro ea CAS: 2646-71-1
Foromo ea molek'hule: C21H26N7Na4O17P3
Boima ba 'mele: 833.35
Bohloeki: ≥97% (HPLC)
Ponahalo: Phofo e tšoeu ho isa ho e tšoeu
Solubility: Ho qhibiliha ha metsi ka bolokolohi (50 mg/mL)
Likarolo tsa Bohlokoa
- Bohloeki bo Phahameng & Botsitso
- E entsoe ka bohloeki ba ≥97%, e netefatsa ts'ebetso e tšepahalang litekong tse hlokolosi tsa lik'hemik'hale.
- E tsitsitse ho -20 ° C ha e bolokiloe e omme ebile e sirelelitsoe khanya; Litharollo tse lokiselitsoeng esale pele li ka tlosoa 'me tsa bolokoa ho -20 ° C bakeng sa likhoeli tse 1-2.
- Likopo tse pharaletseng
- Electron Donor: E sebetsa e le cofactor bakeng sa oxidoreductases, ho akarelletsa le nitric oxide synthase le thioredoxin reductase.
- Biosynthesis: E bohlokoa bakeng sa lipid, cholesterol, le nucleotide synthesis ka karabelo e fokotsang.
- Tšireletso ea Antioxidant: E sireletsa lisele ho tsoa mefuteng ea oksijene e sebetsang (ROS) ka ho boloka maemo a fokotsehileng a glutathione.
- Li-Reagents tsa Tlhahlobo: E sebelisoa litekong tsa enzymatic bakeng sa lipatlisiso tsa bongaka le nts'etsopele ea meriana.
- Thepa ea Optical
- Litlhōrō tsa ho monya ha UV ho 260 nm (ε = 15.0 × 10³ L·mol⁻¹·cm⁻¹) le 340 nm (ε = 6.3 × 10³ L·mol⁻¹·cm⁻¹), e loketseng spectrophotometric quantification
Polokelo & Ho Tšoara
- Polokelo:
- Nako e khuts'oane: 2-8 ° C ka har'a lijana tse sa keneleng moea, tse sirelelitsoeng leseli .
- Nako e telele: -20 ° C maemong a desiccated; qoba lipotoloho tsa ho qhibiliha ha leqhoa .
- Boitokisetso:
- Hlophisa hape ka li-buffers tsa alkaline (mohlala, 10 mM NaOH) bakeng sa botsitso bo nepahetseng; Litharollo tse nang le asiti li senya NADPH ka potlako.
- Centrifuge lyophilized powder ka 2,000-10,000×g pele e sebelisoa ho netefatsa homogeneity.
Polokeho le Boikamahanyo
- Tšebeliso e Reriloeng: Bakeng sa merero ea lipatlisiso feela. Eseng bakeng sa ho hlahloba mafu, ho phekola, kapa ho sebelisoa ke batho .
- Mehato ea Tšireletso:
- Apara lijase tsa laboratori, liatlana, le tšireletso ea mahlo ha u sebetsana le tsona.
- E seng kotsi tlasa melaoana e tloaelehileng ea lipalangoang (likarolo tsa UN NONH) .
Hobaneng o Khetha β-NADPH ea Rōna?
- Maemo a Lefatše: E hlahisitsoe tlas'a FSSC22000 le litsamaiso tsa taolo ea boleng tse lumellanang le FDA.
- Tšehetso ea Theknoloji: E tšehetsoa ke tšebelisano 'moho le litsi tse etelletseng pele (mohlala, Univesithi ea Harvard, CAS) bakeng sa likopo tsa maemo a holimo .
- Sephutheloana se Tloaelehileng: E fumaneha ka bongata ba 10 mg ho isa ho 1 g ho lumellana le litlhoko tse fapaneng tsa liteko.
Mantsoe a bohlokoa: β-NADPH, Coenzyme II e fokotsehile,CAS 2646-71-1, mofani oa elektronike, oxidoreductase cofactor, NADPH tetrasodium letsoai
