Waikawa Oleanolic i te mea he toru triterpenoids pentacyclic taratahi i Gentianaceae tipu o Swertia mileensis te tarutaru privet ranei hua katoa, me te tinana noa, me glycosides i roto i maha ngā tipu. Waikawa Oleanolic he whānui ngā tūmomo i roto i te tipu, me te ihirangi toharite ko te 0.2% - 2%. Cucurbitaceae ihirangi tiketike ko 1.5% ~ i puwhenua te raro 2%, privet ihirangi hua o 0.6% ~ 0.7% .Oleanolic waikawa ko te ahua o te pūhui triterpenoid pentacyclic taratahi i te hua o te puninga Asteraceae, Syzygium sylvestris, Ligustrum ranei lucidum. Ko te adjuvant mate ate me te hauora whakamahia mō te mate whakaora. He pānga kitea i runga i te whakaiti aminotransferase alanine me te waikawa yellowing.Oolic ko te triterpenoids pentacyclic taratahi i te hua o te swertia chinensis fructus ranei ligustris i te tipu gentianaceae whakapeka jaundice kakā. Kei te kitea whānuitia waikawa Oleanolic i roto i te tipu, me te ihirangi whānui o 0.2% ~ 2% [1]. Ko te ihirangi o te raro o te ipu i ~ 2% 1.5%, me te ihirangi o te hua o ligustris fructus ko 0.6% ~ 0.7%. Waikawa Oleanolic he karaihe ma acicular (waihā), koreami me akeake. He mea pokarekare, ki te waikawa me nga turanga. Rewa mata o 308 ~ 310 ℃, [Arepa] 20 d + 73.3 ° (c = 0.15, chloroform, rewakore i roto i te wai, wairewa i roto i te waihā mewaro, te waihā, Ethyl Etere, ko te triterpenoid ka puta noa, whānui tohaina hauwaiwaro me te waikawa chloroform.Oleanolic i roto i ngā tipu kai, me te rongoa, e pā ana ki te waikawa betulinic. ka taea te kitea te reira i roto i Phytolacca Americana (pokeweed American), ko Syzygium spp, kāriki, me ētahi atu ko āhua kore-paitini, hepatoprotective, me ngā whakaaturanga antitumor me ngā āhuatanga patu huaketo.
ko waikawa Oleanolic kitea ki te whakaatu kaha mahi anti-HIV, i whakamahia te waikawa betulinic pūhui e pā ana ki te hanga i te tuatahi tarukino matuatanga inhibitor arumoni. i te tuatahi ako te reira, ka herea ki te maha tipu, tae atu Rosa woodsii (rau), Prosopis glandulosa (rau me manga), Phordendron juniperinum (tipu katoa), Syzygium claviflorum (rau), Hyptis capitata (tipu katoa), ko Ternstromia gymnanthera (wāhanga irirangi). Ētahi atu momo Syzygium tae atu java aporo (Syzygium samarangense) ka whakatika aporo s roto i te reira.
Ingoa Hua: Oleanolic Waikawa 98 %
Whakapūtātanga: 98% i te HPLC
Botanic Source:'Ōleá Europea Tango
Chemical Ingoa: (3β) -3-Hydroxyolean-12-en-28-oic waikawa
CAS No: 508-02-1
Wāhanga whakamahia: Leaf
Tae: paura White ki kakara āhuatanga, me te reka
Tūnga koiora kua: koiora kua Free
Tarapēke: i roto i te 25kgs pahu muka
Storage: Kia mau ipu āu i raumati, wahi maroke, Puritia atu i te marama kaha
Shelf Life: 24 marama i te rā o te hanga
What is oleanolic acid?
Oleanolic acid (OA), a natural hydroxyl pentacyclic triterpenoid acid (HPTA) similar to betulinic acid, ursolic acid; it has benefits like antibacterial, anti-inflammatory, antitumor activities.
Where can you find oleanolic acid?
Oleanolic acid has used traditionally for the treatment of various diseases,you can find it in food and plants widely.
Some fruits like apples, pomegranate, lemon, bilberries, olives contain oleanolic acid too.
| Herb Name | Oleanolic acid content | Test method |
| Ligustrum lucidum Ait | 0.8028% | HPLC |
| Verbena Officinalis L | 0.071%-0.086% | HPLC |
| Prunella vulgaris L | 3.47%-4.46% | HPLC |
| Hemsley Chinensis Cogn. | 1.5%～2% | HPLC |
Currently, Chinese herb Hemsley Chinensis Cogn still is the most commercial raw material for extracting oleanolic acid.
Hemsley Chinensis Cogn. Introduction
Hemsleya chinensis Cogn. is a perennial climbing herb, also is traditional Chinese medicine.
Familia: Cucurbitaceae
Tribus: Gomphogyneae
Genus: Hemsleya
Species: H. amabilis
The herb is distributed in provinces Guangxi, Sichuan, Guizhou, Yunnan, Hubei, etc. Born in the forest edge and valley shrubs at an altitude of about 2,000 meters.
Active ingredients: contains Hemslolide Mal、Ma3、H1; Chikusetsusaponin-Iva; dihydro cucurbitacin F-25-acetate; dilydrocucurbitacin F; oleanolic acid-beta-Hlucosyloleanolate; Hemsamabilinin A; Cu-curbitacinⅡb-2-beta-D-glucopyranoside.
Medicinal Values:
Hemsleya chinensis Cogn. is mainly for detoxification, sterilization, anti-inflammatory, strengthening the stomach and relieving pain. Currently, there are extracts powder, or compound preparations such as capsules, tablets, gastrointestinal pills, etc., which are widely used in clinical practice.
Extraction of Oleanolic acid from Hemsleya Chinensis Cogn.
Formulas containing Oleanolic acid in Food supplements
We found that the oleanolic acid used in health supplements mainly comes from three kinds of plant extracts: Loquat leaf extract, Hemsley Chinensis extract, and holy basil extract.
- Holy Basil powder (leaf) (0.4% Ursolic acid and Oleanolic acid, 2.0 mg)
- Holy Basil supercritical CO2 extract (leaf) (Ocimum tenuiflorum Linn.) (2.5% Ursolic acid and Oleanolic acid, 1.5 mg)
- Loquat Extract (fruit) (providing Ursolic acid, Oleanolic Acid) (Standardized to Ursolic acid Per Serving 125mg)
Oleanolic acid VS Ursolic acid
Oleanolic acid (OA) and ursolic acid (UA) are natural triterpenoids that have a similar chemical structure.
These triterpenoids compounds are known to exist in medicinal herbs and foods.
They have many common pharmacological properties: hepatoprotective, anti-inflammatory, antimicrobial, hypoglycemic, antimutagenic, anti-HIV activity, antioxidant, and antifertility activities.
OA and UA difference:
| Ingoa Hua | Oleanolic acid | Ursolic acid |
| CAS NO. | 508-02-1 | 77-52-1 |
| Pentacyclic triterpenes | β-Amyrin | α-Amyrin |
| Herb sources | Loquat leaf, Holy Basil, Rosemary, Olive leaf.etc. | |
| Specifications | 40%,98%powder | 15%,25%,50%,98%powder |
| Appearance(color and odor) | 40% light yellow98%white powderodorless | 15%-50% brown-yellow or yellow98%white powderCharacteristic |
| Distinguishing | IR:(1355~1392cm-1) two peaks(1245~1330cm-1) three peaksNMR:δ(C12)122.1,δ(C13)143.4 | (1355~1392cm-1) three peaks(1245~1330cm-1) three peaksδ(C12)125.5,δ(C13)138.0 |
| Derivatives | Oleanolic sodium saltOleanolic acid phosphate disodium salt3-oxo oleanolic acid
bardoxolone methyl (CDDO-Me) |
Ursolic sodium salt and its dicarboxylic acid half ester derivativesUrsolic acid ketene derivatives3-carbon ursolic acid
3-acetoxyursolic acid |
| Potential anticancer | UA is more popular than OA. | |
Biological Activities of Oleanolic Acid
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Anti-Tumour/Anti-Cancer Effects
Inhibitory effect of oleanolic acid on hepatocellular carcinoma via ERK-p53-mediated cell cycle arrest and mitochondrial-dependent apoptosis
– by Xin Wang, Hua Bai, etc. Researchers
OA exhibited an inhibitory effect on HCC through induction of apoptosis and cell cycle arrest both in transplanted tumors and in HepG2 cells.
OA induced apoptosis through the mitochondrial pathway, evidenced by inhibition of Akt/mammalian target of rapamycin pathway.
OA induced G2/M cell cycle arrest through p21-mediated down-regulation of cyclin B1/cdc2.
OA demonstrated significant antitumor activities in HCC in vivo and in vitro models. These data provide new insight into the mechanisms underlying the antitumor effect of OA.
In addition, research shows that OA and its derivative Oleanolic acid methyl ester also have effects on breast cancer, lung cancer, bladder cancer, cervical cancer, pancreatic cancer cells…
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Antimicrobial Activity
OA is expected to possess antimicrobial activity against a wide range of pathogens for it plays a vital role in defending against pathogens in plants.
OA showed moderate activity against Staphylococcus aureus and Bacillus Thuringiensis at 62.5 µg/mL and Escherichia coli, Salmonella Enterica, and Shigella dysenteriae at 31.2 µg/mL minimum inhibitory concentration (MIC).
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Hepatoprotective Ability
One of the notable bioactivities of OA is the protection of the liver against toxicity and is currently being used as an over the hepatic counter drug in China.
In Wistar albino rats, OA from Flaveria Trinervia was used and had a significant protective effect on ethanol-induced liver toxicity by restoring the hepatotoxic serum marker enzyme levels. This study suggested the antioxidant ability of OA as another possible mechanism of its hepatoprotective ability.
Oleanolic Acid and its derivatives
Oleanolic Acid Clinical Trials
Oleanolic acid (sourced from olive), there are about 500 registered clinical trials, showed it had beneficial effects in clinical trials on chronic kidney disease, diabetes mellitus type 2, and some inflammatory conditions such as arthritis.
Most popular derivatives in clinical trials are bardoxolone methyl (CDDO-Me). CDDO-Me was evaluated in tumor biopsies, and it might be able to play a role in the treatment of chronic kidney disease, it is currently being assessed in effects on hypertension.
Chinese Pharmacopoeia Standard of Oleanolic Acid
| Ingoa Hua | Oleanolic Acid |
| Tāutunga | (1)Take 30mg of this product, put it in a test tube, add 3ml of chloroform to dissolve, add two drops of sulfuric acid, shake for 5 minutes, the chloroform layer is purple-red. |
| (2)Take about 20mg of this product, add 1ml of acetic anhydride, dissolve it with a slight heat, add sulfuric acid to the purple color, and darken after placing. | |
| (3)Take about 10mg of this product, add vanillin glacial acetic acid solution (take vanillin 0.5g, add 10ml of glacial acetic acid to dissolve, that is) 0.2ml, add 0.8ml of perchloric acid, and heat it for several minutes in a water bath. Fuchsia, add 2ml of ethyl acetate, purple-red dissolved in ethyl acetate, placed without discoloration. | |
| (4)The infrared absorption spectrum of this product should be consistent with the control spectrum. | |
| Determination of assay | Take 0.15g of this product, accurately weigh it, add 30ml of ethanol, shake it, heat it in a warm water bath to dissolve, let cool to room temperature, add 3 drops of phenolphthalein indicator solution, make potassium hydroxide solution with ethanol (0.05mol/L) Titrate immediately and correct for blank test. Potassium hydroxide solution (0.05 mol/L) per 1 ml of ethanol corresponds to 22.84 mg of C30H 48e 3. |
Oleanolic acid recommend dosage
According to the Chinese Pharmacopoeia Standard, Oleanolic acid Oral dose is 20~80mg per time,60~240mg per day.
Oleanolic acid side effects
Oleanolic acid used as an over the counter (OTC) hepatoprotective drug for decades in China.
If overdose or incorrect, a small number of patients have dry mouth, diarrhea, upper abdominal discomfort, and can disappear after symptomatic treatment.
Please consult your physician or pharmacist before using this product.
taumahi:
Ko āhua kore-paitini, antitumor, a hepatoprotective, me te whakaahua i ngā āhuatanga patu huaketo waikawa 1.Oleanolic.
Kitea waikawa 2.Oleanolic i ki te whakaatu kaha mahi anti-HIV.
Waikawa 3.Oleanolic Ko te paruru nui o ngā pūtau ki oxidative me te ahotea electrophile.
Waikawa 4.Oleanolic He pānga nui i runga i trating kakā huaketo, whakapeka kakā icteric me kakā.
Taupānga:
1. Applied i mara kai, taea mahi reira rite rauemi raw o tea ki te whakaiti i maremare;
2. Applied i mara rongoā, riro te reira i te tarukino anti-mate pukupuku hou ki iti paitini;
3. Applied i mara Paku, ka taea e fakalongomo'ui'i reira tohanga toto, me te tango te inu.
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